AKT3 PE Antibody

• Catalog Number: orb344550
• Category: Antibodies
• Description: AKT3 PE antibody (RPE)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 25F6.F6.D8
• Tested applications: DOT, ELISA
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein.
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IF: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from tissue culture by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
• Conjugation: RPE
• UniProt ID: Q9Y243
• NCBI: NP_001193658.1
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Buffer/Preservatives: None
• Alternative names: Mouse anti-AKT3 antibody PE conjugation, phycoeryt
• Note: For research use only
• Application notes: Anti-AKT3 PE Antibody has been tested by ELISA and dot blot and is suitable for Flow Cytometry, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry of Mouse Anti-AKT3 antibody. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT-3 antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10000 for 1 h at RT. Localization: AKT3 is nuclear and occasionally cytoplasmic. Staining: AKT3 as precipitated brown signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of Mouse Monoclonal anti AKT3 Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH6.2. Pressure Cooker. Primary antibody: anti-AKT3 at 2 ug/ml for 1.5 hour @ room Temp. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45" RT.

Western Blot of Mouse Anti-AKT3 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.

Western Blot of Mouse anti-AKT3 antibody. Lane 1: Control. Lane 2: Rapa. Lane 3: T50. Lane 4: T250. Lane 5: Control. Lane 6: Rapa. Lane 7: T50. Lane 8: T250. Lane 9: AKT3 null. Load: 35 µg per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.

Western Blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.