AKT2 Antibody

SKU: orb345418

Description

AKT2 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4000 - 1:16000, WB: 1:1000
• Reactivity: Human, Mouse
• Application Notes:
  This antibody was tested by ELISA and immunoblotting and was found to be reactive with both unphosphorylated and phosphorylated AKT2 in a lysate of HEK293 cells. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This product has been assayed by immunoblot against a HEK293 cell lysate and is reactive at a 1:1,000 dilution showing a band at approximately 60 kDa. A working dilution of 1:4,000 to 1:16,000 is suggested for this product in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature against 0.1 ug of the immunizing peptide. Researchers should determine optimal titers for other applications.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near aa 450-475 (of 481) of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: AKT2
• Purity: This affinity purified antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-AKT2 antibody, AKT 2, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta

Western Blot of Rabbit anti-AKT2 antibody. Lane 1: HEK293 lysate [control]. Lane 2: HEK293 lysate pretreated for 15 min with 25 uM LY294002. Load: 20 mg/lane. Primary antibody: AKT2 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed: phosphorylated (top arrow) and the non-phosphorylated (bottom arrow) forms of endogenous Akt2. Other band(s): nonspecific.

Western Blot of Rabbit anti-AKT2 antibody. Lane 1: mouse skeletal muscle tissue lysates. Lane 2: mouse fat tissue lysates. Lane 3: mouse liver tissue lysates. Load: 35 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Western Blot of Rabbit Anti-AKT2 antibody. Load: 20 mg/lane. Lane 1: HEK293 lysate [Control]. Lane 2: HEK293 lysate + 15 min 4OHT (tamoxifen). Lane 3: HEK293 lysate + 30 min 4OHT (tamoxifen). Lane 4: HEK293 lysate pretreated for 15 min with 25 uM LY294002. Lane 5: HEK293 lysate + 15 min 4OHT (tamoxifen) lysate pretreated for 15 min with 25 uM LY294002. Lane 6: HEK293 lysate + 30 min 4OHT (tamoxifen) lysate pretreated for 15 min with 25 uM LY294002. Primary antibody: Anti-AKT2 Antibody at 1:1000 for 1h at room temperature. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Observed size: Endogenous Akt2 ~60kDa, myristoylated construct ~110kDa. Other band(s): This treatment has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2.

Quick Database Links

Gene Symbol

AKT2

UniProt

P31751

RefSeq (Protein):NP_001317440.1