AKT2 antibody

• Catalog Number: orb345006
• Category: Antibodies
• Description: AKT2 antibody
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 16G11.E8
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
• Concentration: 1mg/ml
• Dilution range: ELISA: 1:2,000 - 1:10,000, FC: User Optimized, IHC: 20 µg/mL, WB: 1:500- 1:2000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
• Conjugation: Unconjugated
• UniProt ID: P31751
• NCBI: NP_001317440.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rat anti-Anti-AKT2 antibody, AKT 2 antibody, AKT-2
• Note: For research use only
• Application notes: Anti-AKT2 Antibody is tested for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GST Tagged recombinant AKT3. Load: 25 ng per lane using AKT2 antibody