AKT2 Antibody

SKU: orb345006

Description

AKT2 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IHC: 20 µg/mL, WB: 1:500- 1:2000
• Reactivity: Human
• Application Notes:
  Anti-AKT2 Antibody is tested for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 16G11.E8
• Immunogen: Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
• Target: AKT2
• Purity: Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 was purified from concentrated tissue culture supernate by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rat anti-Anti-AKT2 antibody, AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta

Flow Cytometry of Rat anti-AKT2 Allophycocyanin antibody. Cells: Jurkat Cells. Stimulation: none. Primary antibody: Allophycocyanin AKT2 antibody at 1.0 µg/ml for 20 min at 4°C.

Immunohistochemistry of Rat monoclonal anti AKT2 Antibody in human breast carcinoma. Tissue: Human Breast Cancer. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH6.2. Pressure Cooker. antibody: anti-AKT2 at 2 ug/ml for 1.5 hour @ room temp. Secondary Ab: mouse anti-rat at 1:50 for 45" RT.

Western Blot of Rat Anti-AKT2 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT-2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rat secondary antibody at 1:20000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.

Western Blot of Rat anti-AKT2 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase rat secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none.

Quick Database Links

Gene Symbol

AKT2

UniProt

P31751

RefSeq (Protein):NP_001317440.1