AKT2 Antibody

SKU: orb1429335

Description

Rabbit polyclonal antibody to AKT2.

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB22732
• Immunogen: Recombinant Protein
• Target: AKT2
• Molecular Weight: 55769

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, AKT2

AKT2 Antibody flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of AKT2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of AKT2 Antibody in mouse brain tissue lysates (35 ug/lane). AKT2 (arrow) was detected using the purified Pab.

Western blot analysis of AKT2 Antibody in NCI-H460 cell line lysates (35 ug/lane). AKT2 (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

AKT2

UniProt

P31751

RefSeq (Protein):NP_001617.1