AKT2 Antibody

• Catalog Number: orb1263522
• Category: Antibodies
• Description: AKT2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the Central region of human AKT2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: AKT2
• UniProt ID: P31751
• NCBI: P31751
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: RAC-beta serine/threonine-protein kinase, Protein
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AKT2 using rabbit polyclonal AKT2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2).

Western blot analysis in 293 cell line lysates (35 ug/lane).

Confocal immunofluorescent analysis of AKT2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AKT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.