AKT2 Antibody

SKU: orb1263521

Description

AKT2 Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, WB
• Reactivity: Human, Mouse
• Application Notes:
  For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This AKT2 antibody is generated from rabbits immunized with human AKT2 recombinant protein.
• Target: AKT2
• Molecular Weight: 56 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, AKT2

Western blot analysis of AKT2 Antibody in NCI-H460 cell line lysates (35 ug/lane)

Western blot analysis of AKT2 Antibody in mouse brain tissue lysates (35 ug/lane)

Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of AKT2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

AKT2

UniProt

P31751

RefSeq:P31751