AKT2 Antibody [8G3B6], KO Validated

• Catalog Number: orb1274477
• Category: Antibodies
• Description: AKT2 Antibody [8G3B6], KO Validated
• Target: AKT2
• Clonality: Monoclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Immunogen: Recombinant protein of human AKT2
• UniProt ID: P31751
• MW: Observed: 60kDa
• Tested applications: ChIP, ICC, IF, IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100ICC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:20 - 1:50ChIP: 1:20 - 1:100
• Antibody Type: Primary Antibody
• Clone Number: [8G3B6]
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: rac protein kinase beta, Murine thymoma viral (v-a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts from normal (control) and AKT2 knockout (KO) 293T cells, using AKT2 antibody (orb1274477) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.