AKT1 AKT2 AKT3 Antibody

SKU: orb1253702

Description

AKT1 AKT2 AKT3 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y (p) -L-A) derived from Human AKT1/AKT2/AKT3.
• Target: AKT1 AKT2 AKT3
• Molecular Weight: 60 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, PKD, PKD1, PRKCM, RAC-PK-alpha, Protein kinase B

Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312).

Quick Database Links

Gene Symbol

AKT1 AKT2 AKT3

UniProt

Q9Y243, P31749, P31751

RefSeq (Protein):NP_001617.1, NP_001014431.1, NP_005456.1