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Catalog Number | orb1238077 |
---|---|
Category | Antibodies |
Description | AIFM2 (IN) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, WB |
Predicted Reactivity | Bovine, Equine, Primate |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Anti-AIFM2 antibody ( orb1238077) was raised against a peptide corresponding to 14 amino acids near the center of human AIFM2. The immunogen is located within 130-180 amino acids of AIFM2. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 41kDObserved: 40 kD |
Target | AIMF2 |
UniProt ID | Q9BRQ8 |
NCBI | Q9BRQ8 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | AIMF2 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
WB Validation in Human Mouse and Rat Cell Lines. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238077, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
WB Validation in Mouse Skeletal Muscle. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238077, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
WB Validation in Rat Tissues. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238077, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
Immunofluorescence Validation of AIFM2 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with orb1238077 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of AIFM2 in Human Brain. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-AIFM2 antibody (orb1238077) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of AIFM2 in Mouse Brain. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AIFM2 antibody (orb1238077) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of AIFM2 in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AIFM2 antibody (orb1238077) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |