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AIFM2 (IN) Antibody

Catalog Number: orb1238077

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1238077
CategoryAntibodies
DescriptionAIFM2 (IN) Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IF, IHC, WB
Predicted ReactivityBovine, Equine, Primate
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenAnti-AIFM2 antibody ( orb1238077) was raised against a peptide corresponding to 14 amino acids near the center of human AIFM2. The immunogen is located within 130-180 amino acids of AIFM2.
Antibody TypePrimary Antibody
Concentration1 mg/mL
Form/AppearanceLiquid
ConjugationUnconjugated
MWPredicted: 41kDObserved: 40 kD
TargetAIMF2
UniProt IDQ9BRQ8
NCBIQ9BRQ8
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesAIFM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Alternative namesAIMF2
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
AIFM2 (IN) Antibody

WB Validation in Human Mouse and Rat Cell Lines. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238077, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.

AIFM2 (IN) Antibody

WB Validation in Mouse Skeletal Muscle. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238077, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.

AIFM2 (IN) Antibody

WB Validation in Rat Tissues. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238077, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.

AIFM2 (IN) Antibody

Immunofluorescence Validation of AIFM2 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with orb1238077 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

AIFM2 (IN) Antibody

Immunohistochemistry Validation of AIFM2 in Human Brain. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-AIFM2 antibody (orb1238077) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

AIFM2 (IN) Antibody

Immunohistochemistry Validation of AIFM2 in Mouse Brain. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AIFM2 antibody (orb1238077) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

AIFM2 (IN) Antibody

Immunohistochemistry Validation of AIFM2 in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AIFM2 antibody (orb1238077) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

  • AIFM2 polyclonal antibody [orb1678077]

    IF,  IHC,  WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 50 μl