AIMF2 Antibody

SKU: orb1238078

Description

AIMF2 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Primate

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-AIFM2 antibody ( orb1238078) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human AIFM2. The immunogen is located within the last 50 amino acids of AIFM2.
• Target: AIMF2
• Molecular Weight: Predicted: 41kDObserved: 41 kD
• Purification: AIMF2 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AIMF2

WB Validation in Human Mouse and Rat Cell Lines. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.

WB Validation in Mouse Tissues. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.

WB Validation in Rat Tissues. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.

Immunofluorescence Validation of AIFM2 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with orb1238078 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunohistochemistry Validation of AIFM2 in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of AIFM2 in Mouse Spleen. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of AIFM2 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Quick Database Links

Gene Symbol

AIMF2

UniProt

Q9BRQ8

RefSeq:Q9BRQ8