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Item 1 of 7
AIFM2 (CT) Antibody
Catalog Number: orb1238078
| Catalog Number | orb1238078 |
|---|---|
| Category | Antibodies |
| Description | AIFM2 (CT) Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Predicted Reactivity | Bovine, Equine, Primate |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Anti-AIFM2 antibody ( orb1238078) was raised against a peptide corresponding to 12 amino acids near the carboxyl terminus of human AIFM2. The immunogen is located within the last 50 amino acids of AIFM2. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Predicted: 41kDObserved: 41 kD |
| Target | AIMF2 |
| UniProt ID | Q9BRQ8 |
| NCBI | Q9BRQ8 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | AIFM2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Alternative names | AIMF2 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
WB Validation in Human Mouse and Rat Cell Lines. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
WB Validation in Mouse Tissues. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
WB Validation in Rat Tissues. Loading: 15 µg of lysate. Antibodies: AIFM2 orb1238078, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjuate at 1:10000 dilution.
Immunofluorescence Validation of AIFM2 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling AIFM2 with orb1238078 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunohistochemistry Validation of AIFM2 in Human Testis. Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of AIFM2 in Mouse Spleen. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry Validation of AIFM2 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AIFM2 antibody (orb1238078) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
