Actin Antibody (pan muscle)

• Catalog Number: orb385922
• Category: Antibodies
• Description: Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. An actin protein's mass is roughly 42-kDa and it is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. It can be present as either a free monomer called G-actin (globular) or as part of a linear polymer microfilament called F-actin (filamentous), both of which are essential for such important cellular functions as the mobility and contraction of cells during cell division. [Wiki]This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PMAC1-1
• Tested applications: IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: An amino acid sequence common among human muscle actins was used as the immunogen for the Muscle Actin antibody.
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Muscle Actin antibody is available for research use only.
• UniProt ID: P62736, P68133, P68032
• Storage: Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Muscle Actin antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human leiomyosarcoma using Muscle Actin antibody

Immunohistochemical staining of Human rhabdomyosarcoma using Muscle Actin antibody

Immunohistochemical staining of Rat skeleton muscle using Muscle Actin antibody

Immunohistochemical staining of Rat heart using Muscle Actin antibody

Western blot analysis of Human skeletal muslce using Muscle Actin antibody