Actin Antibody (pan muscle)

• Catalog Number: orb749983
• Category: Antibodies
• Description: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Immunogen: An amino acid sequence common among human skeletal, cardiac and smooth muscle actins was used as the immunogen for the pan Muscle Actin antibody.
• UniProt ID: P68032, P62736, P68133
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Thomsen-Friedenreich Antigen antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: MSA/953
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cardiovascular Disease, Epigenetics
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with pan Muscle Actin antibody (clone MSA/953).

IHC: Formalin-fixed, paraffin-embedded human leiomyosarcoma stained with pan Muscle Actin antibody (clone MSA/953).

IHC: Formalin-fixed, paraffin-embedded rat stomach stained with pan Muscle Actin antibody (clone MSA/953).

IHC: Formalin-fixed, paraffin-embedded rat lung stained with pan Muscle Actin antibody (clone MSA/953).

IHC: Formalin-fixed, paraffin-embedded rat heart stained with pan Muscle Actin antibody (clone MSA/953).

IHC: Formalin-fixed, paraffin-embedded rat skeletal muscle stained with pan Muscle Actin antibody (clone MSA/953).

Western blot of human skeletal muscle tissue lysate using pan Muscle Actin antibody (clone MSA/953).