ACTA2 Antibody

SKU: orb1248759

Description

ACTA2 Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in Human, Mouse and Rat Duodenum lysates and in lysates of cell lines HeLa and NIH3T3, (calculated MW of 42kDa according to Human NP_001604, Mouse NP_031418.1 and Rat NP_112266.1). Recommended concentration: 0.1-1µg/mL. Primary incubation 1 hour at room temperature.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: EEEDSTALVC
• Target: ACTA2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACTA2, actin, alpha 2, smooth muscle, aorta, OTTHUMP00000020042, ACTSA, AAT6, ACTSA, alpha 2 actin, alpha-cardiac actin, growth-inhibiting gene 46, smooth muscle alpha-actin,

orb1248759 (0.3 ug/ml) staining of HeLa (A) and (0.5 ug/ml) NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248759 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

orb1248759 (3.5 ug/ml) staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining. This data is from a previous batch, not on sale.

orb1248759 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody. This data is from a previous batch, not on sale.

orb1248759 (0.1 ug/ml) staining of Human (A), (0.3 ug/ml) Mouse (B) and Rat (C) Duodenum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

ACTA2

UniProt

P62736

RefSeq (Protein):NP_001135417.1, NP_001604.1