ACHE Antibody

• Catalog Number: orb1248236
• Category: Antibodies
• Description: ACHE Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: QFDHYSKQDRCSDL
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in HepG2 and Jurkat cell lysates and approx. 65-70kDa in Daudi and HeLa cell lysates (calculated MW of 67.8kDa according to NP_000656.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ACHE
• UniProt ID: P22303
• NCBI: NP_000656.1, NP_001269378.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ACHE, YT, acetylcholinesterase (YT blood group), A
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in HepG2 and Jurkat cell lysates and approx. 65-70kDa in Daudi and HeLa cell lysates (calculated MW of 67.8kDa according to NP_000656.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248236 (1 μg/ml) staining of HepG2 (A), Daudi (B) and (0.3 ug/ml) HeLa (C) and Jurkat (D) cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

orb1248236 (0.3 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248236 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic and nuclear/Vesicle staining.

orb1248236 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line).