H4K5AC Antibody

SKU: orb1259609

Description

H4K5AC Antibody

Images & Validation

• Tested Applications: ChIP, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:20 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1).
• Target: H4K5AC
• Molecular Weight: Observed: 11kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody (orb1259609) at 1:1000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat brain using H4K5AC antibody (orb1259609) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human tonsil using H4K5AC antibody (orb1259609) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using H4K5AC antibody (orb1259609) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using H4K5AC antibody (orb1259609) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

H4K5AC

UniProt

P62805