KAT1 / HAT1 Rabbit mAb [KD Validated]

SKU: orb1950557

Description

Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution range: WB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human KAT1 / HAT1
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Disclaimer: For research use only

Alternative Names

hat1; KAT1;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of KAT1 / HAT1 expression in (1) MCF7 cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.

Quick Database Links

UniProt

O14929