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Description
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic acetylated peptide around K12 of human Histone H4 (NP_001029249.1). |
| Target | H4K12ac |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunohistochemistry of paraffin-embedded human appendix using Acetyl-Histone H4-K12 antibody (orb1260041) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H4-K12 antibody (orb1260041) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using Acetyl-Histone H4-K12 antibody (orb1260041) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spinal cord using Acetyl-Histone H4-K12 antibody (orb1260041) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K12 antibody (orb1260041) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K12 antibody (orb1260041) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 ℃ for 18 hours. Blue: DAPI for nuclear staining.
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Protocol Information
H4K12ac Antibody (orb1260041)
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