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Catalog Number | orb517075 |
---|---|
Category | Antibodies |
Description | Acetyl-HIST1H3A (K27) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ChIP, ELISA, ICC, IF, IP, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Peptide sequence around site of Acetyl-Lys (27) derived from Human Histone H3.1 |
Dilution range | WB:1:500-1:5000, IHC-P:1:200-1:500, IF:1:50-1:200, IP:1:200-1:2000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | HIST1H3A |
UniProt ID | P68431 |
Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Buffer/Preservatives | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol |
Alternative names | H3 histone family member E pseudogene antibody; H3 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5ug anti-HIST1H3A or a control normal rabbit IgG. The resulting ChIP DNA was quantified usi
Western blot aanalysis of Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysateusing Acetyl-HIST1H3A (K27) antibody
Immunoprecipitation analysis of HepG2 whole cell lysate using Acetyl-HIST1H3A (K27) antibody
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) using Acetyl-HIST1H3A (K27) antibody
Immunocytochemistry analysis of Hela cells using Acetyl-HIST1H3A (K27) antibody
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