ACAT1 Antibody (C-term)

SKU: orb1929153

Description

ACAT1 Antibody (C-term)

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Monkey

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 45200 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

ACAT, MAT

Western blot analysis of anti-ACAT1 Pab in SK-BR-3 cell line lysates (35 ug/lane).ACAT1 (arrow) was detected using the purified Pab.

All lanes: Anti-ACAT1 Antibody (C-term) at 1:2000 dilution. Lane 1: SW620 whole cell lysates. Lane 2: mouse liver lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45. 2 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ACAT1 Antibody (C-term) at 1:1000 dilution. Lane 1: SK-BR-3 whole cell lysates. Lane 2: HepG2 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45. 2 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

UniProt

P24752