ACAT1 Antibody (C-term)

• Catalog Number: orb1929153
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14644
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 45200 Da
• Target: This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-329 amino acids from the C-terminal region of human ACAT1.
• UniProt ID: P24752
• NCBI: NP_000010.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Acetyl-CoA acetyltransferase, mitochondrial, Aceto
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-ACAT1 Pab in SK-BR-3 cell line lysates (35 ug/lane).ACAT1 (arrow) was detected using the purified Pab.

All lanes: Anti-ACAT1 Antibody (C-term) at 1:2000 dilution. Lane 1: SW620 whole cell lysates. Lane 2: mouse liver lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45. 2 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ACAT1 Antibody (C-term) at 1:1000 dilution. Lane 1: SK-BR-3 whole cell lysates. Lane 2: HepG2 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45. 2 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.