ACAT1 Antibody (C-term)

SKU: orb1787990

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10~50
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-349 amino acids from the C-terminal region of human ACAT1. Antigen Region: 311-349 aa.
• Target: ACAT1
• Molecular Weight: 45200 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-ACAT1 Antibody (C-term) at 1:1000 dilution. Lane 1: HepG2 whole cell lysates. Lane 2: mouse liver lysates. Lane 3: SK-BR-3 whole cell lysates. Lane 4: SW620 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

ACAT1

UniProt

P24752