ACAN Rabbit Polyclonal Antibody

SKU: orb10066

Description

ACAN Rabbit Polyclonal Antibody

Research Area

Cell Biology, Chondrogenesis, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ACAN (101-220/2415aa)
• Target: ACAN
• Molecular Weight: 208/248 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Aggrecan; Aggrecan core protein; Cartilage-specific proteoglycan core protein; CSPCP; Chondroitin sulfate proteoglycan core protein 1; Chondroitin sulfate proteoglycan 1; Aggrecan core protein 2; AGC1; CSPG1; MSK16; PGCA_HUMAN.

Paraformaldehyde-fixed, paraffin embedded Mouse Embryo, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with ACAN Polyclonal Antibody, Unconjugated (orb10066) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with ACAN Polyclonal Antibody, Unconjugated (orb10066) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Embryo, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with ACAN Polyclonal Antibody, Unconjugated (orb10066) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Tissue/cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-Aggrecan1 Polyclonal Antibody, Unconjugated (orb10066) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ACAN

UniProt

P16112

References

Sen-Ei Shai 1 2 3, Yi-Ling Lai 1, Yi-Wen Hung 4, Chi-Wei Hsieh 5, Kuo-Chih Su 6, Chun-Hsiang Wang 6, Te-Hsin Chao 7, Yung-Tsung Chiu 8, Chia-Ching Wu 5 9, Shih-Chieh Hung Chondrogenesis in primitive tracheal neocartilage: insights from 3D-printed silicone grafts in a large-scale animal model Am J Transl Res, (2025)

PubMed: 40092134

Applications

IHC

Reactivity

Porcine

Shai, Sen-Ei et al. Feasibility of in situ chondrogenesis for the entire umbilical cord in preliminary preparation for tracheal graft Am J Transl Res, 13, 1307-1321 (2021)

PubMed: 33841658

Applications

IHC

Reactivity

Porcine