ACAN Rabbit Polyclonal Antibody

• Catalog Number: orb1499427
• Category: Antibodies
• Description: ACAN Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant human ACAN protein (2321-2530/2530aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 222 kDa
• Target: ACAN
• UniProt ID: P16112
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Aggrecan; Aggrecan core protein; Cartilage-specifi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Measured by its binding ability in a indirect ELISA. Immobilized Human ACAN protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human ACAN Antibody, the EC50 is 3071 ng/ml.

Measured by its binding ability in a indirect ELISA. Immobilized Human ACAN protein, His Tag at 2 µg/mL (100 µl/well) can bind Rabbit Anti-Human ACAN Antibody, the EC50 is 402 ng/ml.

Sample: Lane 1: Recombinant human ACAN protein, Primary: Anti-ACAN (orb1499427) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 222 kDa, Observed band size: 27 kDa.