Ac4GalNAz

SKU: orb1734944

Description

The tetraacetylated N-Azidoacetyl-galactosamine (Ac 4GalNAz) provides a non-radioactive alternative for glycoconjugate vi-sualization. It is cell-permeable, intracellularly processed and incorporated instead of its natural monosaccharide counterpart N-Acetylgalactosamine(GalNAc). The resulting Azide-functionalized glycoconjugates can subse-quently be detected via Cu(I)-catalyzed or Cu(I)-free Click Chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin or DBCO-containing Biotin, respectively) for subsequent purification tasks or to introduce fluorescent group (via Azides of fluorescent dyes or DBCO-containing fluorescent dyes, respectively) for subsequent microscopic imaging. Recommended concentration for metabolic labeling: 25-75 M. This concentration range may serve as a starting point for an individual assay set-up.

Images & Validation

• Application Notes:
  Glycoconjugate synthesis monitoring by metabolic labeling.

Key Properties

• CAS Number: 653600-56-7
• MW: Theoretical MW: 430.37 g/mol; Detected MW: 430.13 g/mol
• Purity: mass identification (ESI-MS)
• Formula: C₁₆H₂₂N₄O₁₀
• SMILES: [C@H]1([C@H](C(O[C@@H]([C@@H]1OC(=O)C)COC(=O)C)OC(=O)C)NC(=O)CN=[N+]=[N-])OC(=O)C

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only