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ABHD5 Antibody
Description
Images & Validation
−| Tested Applications | ELISA, IF, IHC, IHC-P, WB |
|---|---|
| Dilution range | IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.2 - 0.6 µg/ml) |
| Reactivity | Canine, Equine, Human, Mammal, Rabbit |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence according to NP_057090.2. |
| Target | ABHD5 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin |
| Concentration | 0.5 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CGI58/ABHD5 Antibody [orb19437]
ELISA, IF, IHC, WB
Canine, Rat
Human, Mouse
Goat
Polyclonal
Unconjugated
100 μgABHD5 Antibody [orb519737]
ELISA, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl, 50 μl

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CGI58 / ABHD5 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

ABHD5 (0.2 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Protocol Information
ABHD5 Antibody (orb1538332)
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