CGI58/ABHD5 Antibody

SKU: orb19437

Description

Goat polyclonal antibody to ABHD5

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.2-0.6 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 40kDa band observed in lysates of cell lines NIH3T3 and A431 (calculated MW of 39.1kDa according to NP_057090.2). Recommended concentration: 0.2-0.6 μg/ml. Additional faint bands of 26kDa and 18kDa were observed. These bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

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Key Properties

• Clonality: Polyclonal
• Target: CGI58 / ABHD5
• Protein Sequence: FPERPDLADQDR
• Molecular Weight: 39.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ABHD5 antibody, anti CGI58 protein antibody, anti aCGI58 antibody, anti bhydrolase domain containing 5 antibody, anti CDS antibody, anti IECN2 antibody, anti MGC8731 antibody, anti NCIE2 antibody

Quick Database Links

Gene Symbol

CGI58 / ABHD5

NCBI Gene ID

51099