CGI58/ABHD5 Antibody

• Catalog Number: orb19437
• Category: Antibodies
• Description: Goat polyclonal antibody to ABHD5
• Target: CGI58 / ABHD5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: FPERPDLADQDR
• RRID: AB_10749094
• MW: 39.1
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.2-0.6 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 40kDa band observed in lysates of cell lines NIH3T3 and A431 (calculated MW of 39.1kDa according to NP_057090.2). Recommended concentration: 0.2-0.6 μg/ml. Additional faint bands of 26kDa and 18kDa were observed. These bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ABHD5 antibody, anti CGI58 protein antibody,
• Note: For research use only
• Entrez: 51099

Western blot analyis of NIH3T3 lysate using ABHD5 antibody

Immunohistochemical staining of Human Kidney using ABHD5 antibody

Immunohistochemical staining of Human Pancreas using ABHD5 antibody

Immunofluorescence analysis of U2OS cells using ABHD5 antibody