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Catalog Number | orb19437 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ABHD5 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, WB |
Reactivity | Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:16000, WB: 0.2-0.6 μg/ml, IHC-P: 3.75 μg/ml |
Conjugation | Unconjugated |
MW | 39.1 |
Target | CGI58 / ABHD5 |
Entrez | 51099 |
Protein Sequence | FPERPDLADQDR |
RRID | AB_10749094 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti ABHD5 antibody, anti CGI58 protein antibody, Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 40kDa band observed in lysates of cell lines NIH3T3 and A431 (calculated MW of 39.1kDa according to NP_057090.2). Recommended concentration: 0.2-0.6 μg/ml. Additional faint bands of 26kDa and 18kDa were observed. These bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
Western blot analyis of NIH3T3 lysate using ABHD5 antibody
Immunohistochemical staining of Human Kidney using ABHD5 antibody
Immunohistochemical staining of Human Pancreas using ABHD5 antibody
Immunofluorescence analysis of U2OS cells using ABHD5 antibody