ABCD3 Antibody

• Catalog Number: orb1246591
• Category: Antibodies
• Description: ABCD3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-PDGREDQKRKGISD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 70kDa band observed in Human Kidney lysates (calculated MW of 75.5kDa according to NP_002849.1). Recommended concentration: 0.2-0.6 ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Testis shows vesicular staining in Sertoli cells. Recommended concentration: 2.5-3.8ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ABCD3
• UniProt ID: P28288
• NCBI: NP_002849.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ABCD3, ATP-binding cassette, sub-family D (ALD), m
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 70kDa band observed in Human Kidney lysates (calculated MW of 75.5kDa according to NP_002849.1). Recommended concentration: 0.2-0.6 ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Testis shows vesicular staining in Sertoli cells. Recommended concentration: 2.5-3.8ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246591 (0.2 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246591 (3.8 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.