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Catalog Number | orb1258088 |
---|---|
Category | Antibodies |
Description | ABCB8 Antibody |
Target | ABCB8 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 469-718 of human ABCB8 (NP_009119.2). |
UniProt ID | Q9NUT2 |
MW | Observed: 80kDa |
Tested applications | IF, IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | ABCB8, EST328128, M-ABC1, MABC1 |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using ABCB8 antibody (orb1258088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human liver damage using ABCB8 antibody (orb1258088) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ABCB8 antibody (orb1258088) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ABCB8 antibody (orb1258088) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ABCB8 Polyclonal Antibody (orb1258088) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |