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Anti-ABCB8 Antibody

Catalog Number: orb377888

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb377888
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ABCB8.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenRecombinant full length protein of human ABCB8
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000, IHC-P: 1:50-100
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetABCB8
Entrez11194, 362302, 74610
UniProt IDQ5RKI8, Q9NUT2, Q9CXJ4
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesMABC1; ATP-binding cassette sub-family B member 8
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-ABCB8 Antibody

Western blot analysis of ABCB8 expression in Hela (A), HepG2 (B), mouse kidney (C), rat heart (D) whole cell lysates. (Predicted band size: 59; 68; 75; 78; 79 kD; Observed band size: 80 kD)

Anti-ABCB8 Antibody

Immunohistochemical analysis of ABCB8 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-ABCB8 Antibody

Immunofluorescent analysis of ABCB8 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    100 μg
  • Anti-ABCB8 [orb1129236]

    WB

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    ELISA,  IHC,  WB

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