ABCB5 Antibody

• Catalog Number: orb1246761
• Category: Antibodies
• Description: ABCB5 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QTQHRNTSKKAQ
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ABCB5
• UniProt ID: Q2M3G0
• NCBI: NP_848654.3, NP_001157413.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ATP-binding cassette, sub-family B (MDR/TAP), memb
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in lysates of cell line A431(calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246761 (0.3 ug/ml) staining of lysates of cell line A431 (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml).