ABCB5 Antibody (N-term)

SKU: orb1930242

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:2000, FC - 1:25
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This ABCB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ABCB5. Antigen Region: 1-30 aa.
• Target: ABCB5 (HGNC:46)
• Molecular Weight: 138641 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATP-binding cassette sub-family B member 5, ABCB5 P-gp, P-glycoprotein ABCB5, ABCB5

Overlay histogram showing HepG2 cells stained (green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western blot analysis of ABCB5 Antibody (N-term) in A375, K562, A2058 and HL-60 cell line lysates (35 ug/lane). ABCB5 (arrow) was detected using the purified Pab.

Flow Cytometry using ABCB5 Antibody (N-Term) on WM852 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer.

Flow Cytometry using ABCB5 Antibody (N-Term) on SkMel-30 cell line. Live cell staining utilized PE-conjugated goat anti-rabbit as a secondary antibody. Analysis was done on an FC500 flow cytometer.

Anti-ABCB5 Antibody (N-term) at 1:2000 dilution + Mouse eyeball lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 138 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ABCB5 Antibody (N-term) at 1:2000 dilution. Lane 1: A375 whole cell lysate. Lane 2: HL-60 whole cell lysate. Lane 3: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 138 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ABCB5 Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: A375 whole cell lysate. Lane 5: A2058 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 138 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

ABCB5 (HGNC:46)

UniProt

Q2M3G0

RefSeq (Protein):NP_848654.3, NP_001157465.1, NP_001157414.1, NP_001157413.1