AADACL1 Antibody

SKU: orb1246762

Description

AADACL1 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, FC, IF
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments gave an approx 26-28kDa band in A431 cell lysates and in Human Hippocampus lysates after 1µg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we currently cannot find an explanation in the literature for the band, given the calculated size of 31.2kDa according to NP_001139749.1Immunofluorescence: Strong expression of the protein seen in the Endoplasmic reticulum of A431 and U2OS cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RTRNSYIKWLDQN
• Target: AADACL1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AADACL1, arylacetamide deacetylase-like 1

orb1246762 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Endoplasmic reticulum staining.

orb1246762 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Endoplasmic reticulum staining.

orb1246762 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

AADACL1

UniProt

Q6PIU2

RefSeq (Protein):NP_001139748.2, NP_001139749.1, NP_065843.3