8-Bromo-cAMP

SKU: orb1694603

Description

8-Bromo-cAMP is a cell-permeable activator of protein kinase A (PKA). This cAMP analog is widely used in biochemical and cell biology research to study PKA signaling, where it has been shown to inhibit proliferation and induce apoptosis in various in vitro cell models.

Research Area

Cell Biology, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 23583-48-4
• MW: 408.1
• Purity: 97.30% (May vary between batches)
• Formula: C₁₀H₁₁BrN₅O₆P
• SMILES: BrC=1N(C=2C(N1)=C(N)N=CN2)[C@H]3[C@H](O)[C@]4([C@](O3)(COP(=O)(O)O4)[H])[H]
• Target: cAMP,Apoptosis,PKA
• Solubility: 1 M NaOH:80 mg/mL (196.03 mM);PBS (pH 7.2):2 mg/mL (4.9 mM)

Bioactivity

• In Vivo:
  In renal denervation rat models, 8-Bromo-cAMP (0.5 mmol/L, perfused via the left renal artery at 0.05 mL/min for 60 min) significantly increased expression of Na⁺-K⁺-ATPase α1 and β1 subunits in the renal cortex, indicating its ability to enhance sodium pump activity and support renal ion transport in vivo.
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• In Vitro:
  In primary rat renal tubular epithelial cells, 8-Bromo-cAMP (0.1 mM, 48 h) significantly increased both mRNA and protein levels of Na⁺-K⁺-ATPase α1 and β1 subunits, and promoted their membrane localization, suggesting enhancement of tubular ion transport via PKA signaling. In NRK-52E rat renal proximal tubule epithelial cells, 8-Bromo-cAMP (10⁻⁶–10⁻³ mol/L, 48 h) significantly increased protein and mRNA levels of Na⁺-K⁺-ATPase α1 and β1 subunits (most prominently at 10⁻⁴ mol/L), and promoted their membrane localization, suggesting regulation via the cAMP/PKA signaling pathway.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

8 bromo Cyclic AMP, 8-Br-Camp, 8bromoCyclic AMP, 8-bromo-Cyclic AMP, 8-Bromo-Cyclic AMP

Compound Identifiers

PubChem CID

32014