8-Bromo-cAMP sodium salt

SKU: orb1300503

Description

8-Bromo-cAMP sodium salt is a cell-permeable, hydrolysis-resistant analog of cyclic AMP that acts as a potent activator of Protein Kinase A (PKA). This research compound is widely used in vitro and in vivo to study cAMP/PKA signaling pathways in cancer biology, demonstrating antitumor properties in various experimental models.

Research Area

Cell Biology, Signal Transduction

Key Properties

• CAS Number: 76939-46-3
• MW: 430.08
• Purity: 99.94% (May vary between batches)
• Formula: C₁₀H₁₀BrN₅NaO₆P
• SMILES: BrC(N([C@H]1[C@@H]([C@@]2([H])[C@](COP([O-])(O2)=O)([H])O1)O)C3=NC=N4)=NC3=C4N.[Na+]
• Target: Apoptosis,PKA
• Solubility: PBS:100 mg/mL (232.51 mM);5% DMSO＋40% PEG300＋5% Tween-80＋50% ddH2O:0.624;H2O:80 mg/mL (186.01 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:128 mg/mL (297.62 mM)

Bioactivity

• Target IC50:
  PKA:0.05 μM(Ka)
• In Vivo:
  METHODS: To evaluate the effect of cAMP/PCA on angiogenesis and VM in CRC cells, 8-Bromo-cAMP sodium salt (60 mg/kg) was intraperitoneally injected into BALB/c mice bearing CT26 tumor tissues once a day for seven days. RESULTS: 8-Bromo-cAMP treatment significantly reduced the number of tumors. 8-Bromo-cAMP treatment inhibited angiogenesis and VM. 8-Bromo-cAMP acted through the cAMP/PKA-ERK pathway and EMT process in CRC.
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• In Vitro:
  METHODS: Endothelial HUVECs were treated with 8-Bromo-cAMP sodium salt (100 µM) for 3 h. Cell migration was detected by Boyden Chamber assay. RESULTS: Increased cell migration was observed when conditioned media from MC3T3-E1 cells exposed to 8-Bromo-cAMP was supplemented with HUVECs. In contrast, conditioned medium from control untreated cells lacked this activity. METHODS: The leukemia cell line AML193 was treated with 8-Bromo-cAMP sodium salt (1 mM) or IL-3 (500 ng/mL) for 1-15 min, and target protein expression levels were detected by Western Blot. RESULTS: In growth-arrested AML193 cells, the maximal effect of IL-3 on Erk1,2 phosphorylation was time-dependent and occurred within a time window of 5-15 min after the addition of growth factors. 8-Bromo-cAMP also induced Erk1,2 phosphorylation in these cells, with a maximal stimulation observable between 1-5 min.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PKA, 8 Bromo cAMP sodium salt, 8-Br-Camp sodium, 8-Br-Camp sodium salt, 8-Bromo-cAMP sodium, 8BromocAMP sodium salt, 8-Bromo-cAMP(sodium salt)

Compound Identifiers

PubChem CID

23712387