53BP1 rabbit pAb

• Catalog Number: orb764427
• Category: Antibodies
• Description: Rabbit polyclonal antibody to 53BP1
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human 53BP1. AA range:1-50
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 213
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-TP53BP1 antibody, anti-Tumor suppressor p53-b
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using 53BP1 Polyclonal Antibody diluted at 1:2000.

Immunofluorescence analysis of HeLa cells, using 53BP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using 53BP1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).