53BP1 (phospho Ser25) rabbit pAb

SKU: orb770311

Description

Rabbit polyclonal antibody to 53BP1 (phospho-Ser25)

Images & Validation

• Tested Applications: ELISA, IF, IHC-P
• Dilution range: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human 53BP1 around the phosphorylation site of Ser25. AA range:10-59
• Molecular Weight: 214kD
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-TP53BP1 antibody, anti-Tumor suppressor p53-binding protein 1 antibody, anti-53BP1 antibody, anti-p53-binding protein 1 antibody, anti-p53BP1 antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 53BP1 (Phospho-Ser25) Antibody.

Immunofluorescence analysis of NIH/3T3 cells, using 53BP1 (Phospho-Ser25) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using 53BP1 (Phospho-Ser25) Antibody. The picture on the right is blocked with the phospho peptide.