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5-Ethynyl-2'-deoxyuridine (5-EdU)
Catalog Number: orb64972
| Catalog Number | orb64972 |
|---|---|
| Category | Small Molecules |
| Description | 5-EdU (5-Ethynyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymi-dine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging. |
| Form/Appearance | solid; Color: white to off-white |
| Purity | ≥ 98% (HPLC) |
| MW | Theoretical MW: 252.23 g/mol; Detected MW: 252.07 g/mol |
| Application notes | DNA synthesis monitoring. Spectroscopic Propertie: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
| Solubility (25°C) | DMSO, up to 200 mM (at room temperature) in 1x PBS or water by heating the obtained suspension for 1 minute by 70 °C |
| CAS Number | 61135-33-9 |
| Formula | C11H12N2O5 |
| SMILES | n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1C[C@@H]([C@H](O1)CO)O |
| Storage | store at -20 °C |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
5-Dibenzylcyclooctyne-2'-deoxyuridine (5-DBCO-dU) [orb532331]
≥ 95% (HPLC)
Theoretical MW: 568.58 g/mol (free acid); Detected MW: 568.20 g/mol (free acid)
C31H28N4O7
10 mg5-Vinyl-2'-deoxyuridine (5-VdU) [orb532301]
≥ 98% (HPLC)
55520-67-7
Theoretical MW: 254.24 g/mol; Detected MW: 254.09 g/mol
C11H14N2O5
10 mg5-Vinyl-2'-deoxyuridine (5-VdU) [orb1734969]
≥ 98% (HPLC)
55520-67-7
Theoretical MW: 254.24 g/mol; Detected MW: 254.09 g/mol
C11H14N2O5
50 mg5-Ethynyl-2'-deoxyuridine (5-EdU) [orb1734893]
≥ 98% (HPLC)
61135-33-9
Theoretical MW: 252.23 g/mol; Detected MW: 252.07 g/mol
C11H12N2O5
5 g5-Ethynyl-2'-deoxyuridine (5-EdU) [orb1734894]
≥ 98% (HPLC)
61135-33-9
Theoretical MW: 252.23 g/mol; Detected MW: 252.07 g/mol
C11H12N2O5
500 mg
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
