5-Ethynyl-2'-deoxyuridine (5-EdU)

• Catalog Number: orb64972
• Category: Tools
• Description: 5-EdU (5-Ethynyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymi-dine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.
• CAS Number: 61135-33-9
• Form/Appearance: solid; Color: white to off-white
• Purity: ≥ 98% (HPLC)
• MW: Theoretical MW: 252.23 g/mol; Detected MW: 252.07 g/mol
• SMILES: n1(cc(c(=O)[nH]c1=O)C#C)[C@H]1C[C@@H]([C@H](O1)CO)O
• Formula: C₁₁H₁₂N₂O₅
• Solubility (25°C): DMSO, up to 200 mM (at room temperature) in 1x PBS or water by heating the obtained suspension for 1 minute by 70 °C
• Storage: store at -20 °C
• Note: For research use only
• Application notes: DNA synthesis monitoring. ; Spectroscopic Propertie: λmax 288 nm, ε 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• Expiration Date: 12 months from date of receipt.