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5-Dibenzylcyclooctyne-2'-deoxyuridine (5-DBCO-dU)
Catalog Number: orb532331
| Catalog Number | orb532331 |
|---|---|
| Category | Small Molecules |
| Description | 5-DBCO-dU (5-Dibenzylcyclooctyne-2'-deoxyuridine) can potentially be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis in proliferating cells. 5-DBCO-dU is cell permeable and may incorporate into replicating DNA instead of its natural analog thymidine. The resulting DBCO-functionalized DNA can subsequently be detected via Cu(I)-free Click Chemistry that offers the choice to: introduce a Biotin group for subsequent purification tasks (via Azides of Biotin); introduce a fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes). |
| Form/Appearance | solid; Color: colorless to slightly yellow |
| Purity | ≥ 95% (HPLC) |
| MW | Theoretical MW: 568.58 g/mol (free acid); Detected MW: 568.20 g/mol (free acid) |
| Application notes | DNA synthesis monitoring (potentially). Spectroscopic Propertie: λmax 289 nm, ε 12.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
| Formula | C31H28N4O7 |
| SMILES | c1(=O)[nH]c(=O)n(cc1C#CCNC(=O)CCC(=O)N1Cc2c(C#Cc3c1cccc3)cccc2)[C@H]1C[C@H](O)[C@H](O1)CO |
| Storage | store at -20 °C |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
For example, if the dosage of your experiment is 10mg/kg, the weight of each animal is 50g, and the volume of the dosing is 100μL, then the working solution concentration of your experiment is 5mg/mL. If the formula you used is 5% DMSO+ 30% PEG300+ 5% Tween 80 + 60% Saline/PBS/ddH2O, the preparation method is as follows:
Mother liquor preparation method: 5mg of drug dissolved in 100μL DMSO (mother liquor concentration is 50mg/mL). Preparation method of in vivo formulation: For 1mL of working solution preparation, take 50μL of DMSO mother liquor, add 300 μL of PEG300 to mix well and clarify, then add 50μL of Tween-80 to mix well and clarify, and finally add 600μL of Saline/PBS/ddH2O to mix well and clarify.Note:
- For Reference Only. Please choose the appropriate dissolution protocol according to your experimental animal and administration method.
- We suggest that in cell experiments, the final concentration of DMSO is not more than 0.1%, in animal experiments, for healthy mice, the final concentration of DMSO is not more than 10%; For nude or frail mice, the final concentration of DMSO is not more than 2%.
