5-Azidomethyl-2'-deoxyuridine (5-AmdU)

SKU: orb532314

Description

5-AmdU (5-Azidomethyl-2'-deoxyuridine) can be used as a replace-ment for BrdU (5-Bromo-2'-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-AmdU is cell permeable and incorporates into peplicating DNA instead of its natural analog thymidine. The resulting azide-functionalized DNA can subsequently be detected via Cu(I)-catalyzed (CuAAC) or Cu(I)-free Click Chemistry that offers the choice to: introduce a Biotin group for subsequent purification tasks (via Alkynes of Biotin or DBCO-functionalized Biotin, respectivly); introduce a fluorescent group for subsequent microscopic imaging (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectivly).

Images & Validation

• Application Notes:
  DNA synthesis monitoring. ; Spectroscopic Propertie: max 263 nm, 9.7 L mmol-1 cm-1.

Key Properties

• Molecular Weight: Theoretical MW: 283.24 g/mol; Detected MW: 283.09 g/mol
• Purity: 90% (HPLC)
• Formula: C₁₀H₁₃N₅O₅
• SMILES: O=c1c(cn(c(=O)[nH]1)[C@@H]1O[C@H](CO)[C@@H](O)C1)CN=[N+]=[N-]

Storage & Handling

• Storage: store at -20 C
• Form/Appearance: solid; Color: white to off-white
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only