4-Hydroxynonenal Antibody

• Catalog Number: orb396586
• Category: Antibodies
• Description: Mouse Anti-4-Hydroxynonenal (4-HNE) Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 12F7
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); ICC/IF (1:50); ELISA (1:1000)
• Conjugation: Unconjugated
• Target: 4-Hydroxynonenal
• Storage: -20°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: 4-Hydroxynonenal Antibody, 4-Hydroxy Nonenal (4-HN
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-511 was sufficient for detection of 4-Hydroxynonenal in 0.5 µg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody, Clone 12F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 μM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alexa Fluor 633 F-Actin stain. (E, F) 4-Hydroxynonenal Antibody. (G, H) Composite.

Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal protein using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 μg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 μg). Lane 4: 4-hydroxy nonenal-BSA (2.0 μg). Lane 5: 4-hydroxy-2-hexenal (0.5 μg). Lane 6: 4-hydroxy-2-hexenal (2.0 μg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.