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Catalog Number | orb248126 |
---|---|
Category | Antibodies |
Description | 14-3-3 proteins are regulators of a diverse group of signaling proteins, including kinases, phosphatases, and transmembrane receptors, and have roles in a wide range of physiological processes including transcription, intracellular signaling, cell cycle control and apoptosis. There are seven 14-3-3 proteins in most mammals: beta, gamma epsilon, zeta, eta, sigma and tau. 14-3-3 sigma, also called Stratifin, is a product of the SFN gene. It is a tumor suppressor gene inactivated via DNA methylation. Reduced or no expression of 14-3-3 sigma is seen in various carcinoma including skin, breast and prostate, suggesting that 14-3-3 gene silencing may be involved in tumor progression. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A recombinant protein fragment from the internal region of 14-3-3 sigma was used as the immunogen for this antibody. The amino acid sequence is common to the ~30kDa and ~20kDa isoforms. |
Dilution range | Western blot: 1-3ug/ml |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This 14-3-3 sigma antibody is available for research use only. |
Entrez | 2810 |
Storage | Aliquot the 14-3-3 sigma antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 1X PBS, pH 7.4 |
Note | For research use only |
Application notes | Provided assay concentrations are suggestions only, 14-3-3 sigma antibody titration may be required for optimal results. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of human samples using 14-3-3 sigma antibody (primary antibody dilution at: at 1 ug/ml)
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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