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14-3-3 sigma/SFN/14 Rabbit Polyclonal Antibody

SKU: orb76310

Description

14-3-3 sigma/SFN/14 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

Tested ApplicationsFC, ICC, IF, IHC, WB
Dilution RangeWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunocytochemistry, 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
ReactivityHuman, Mouse, Rat

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma, identical to the related rat and mouse sequences.
Target14-3-3 protein sigma
Molecular Weight28 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin; SFN; HME1

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14-3-3 sigma/SFN/14 Rabbit Polyclonal Antibody

Western blot analysis of Lane 1: HELA Cell Lysate, Lane 2: A549 Cell Lysate, Lane 3: A549 Cell Lysate, Lane 4: COLO320 Cell Lysate, Lane 5: SE620 Cell Lysate using Stratifin antibody

14-3-3 sigma/SFN/14 Rabbit Polyclonal Antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human T

14-3-3 sigma/SFN/14 Rabbit Polyclonal Antibody
14-3-3 sigma/SFN/14 Rabbit Polyclonal Antibody

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
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FC
Flow Cytometry
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IF
Immunofluorescence
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ICC
Immunocytochemistry
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14-3-3 sigma/SFN/14 Rabbit Polyclonal Antibody (orb76310)

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Available Sizes

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100 μg
$ 450.00
DispatchUsually dispatched within 2-4 weeks
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