ZXDC Antibody / ZXD-like zinc finger protein

• Catalog Number: orb1823649
• Category: Antibodies
• Description: ZXDC cooperates with CIITA to promote transcription of MHC class I and MHC class II genes [UniProt].
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human ZXDC protein was used as the immunogen for the ZXDC antibody.
• UniProt ID: Q2QGD7
• Tested applications: FACS, IF, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: PCRP-ZXDC-2B5
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Immunofluorescent staining of PFA-fixed human HeLa cells with ZXDC antibody (clone PCRP-ZXDC-2B5). followed by goat anti-mouse IgG-CF488 (green); Red = CF640R phalloidin.

IHC staining of FFPE human prostate tissue with ZXDC antibody (clone PCRP-ZXDC-2B5). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Flow cytometry testing of PFA-fixed human HeLa cells with ZXDC antibody (clone PCRP-ZXDC-2B5) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using ZXDC Mouse Monoclonal (PCRP-ZXDC-2B5). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.

SDS-PAGE analysis of purified, BSA-free ZXDC antibody (clone PCRP-ZXDC-2B5) as confirmation of integrity and purity.