ZP4 Antibody (C-term)

• Catalog Number: orb1937242
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32332
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:2000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 59400 Da
• Target: This ZP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human ZP4.
• UniProt ID: Q12836
• NCBI: NP_067009.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Zona pellucida sperm-binding protein 4, Zona pellu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-ZP4 Antibody (C-term) at 1:2000 dilution + NCCIT whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ZP4 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ZP4 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ZP4 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the ZP4 antibody detected the ZP4 protein (arrow).