ZNRF3 Antibody

• Catalog Number: orb1259198
• Category: Antibodies
• Description: ZNRF3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ZNRF3 (NP_115549.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 120kDa
• Target: ZNRF3
• UniProt ID: Q9ULT6
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: BK747E2.3, RNF203, E3 ubiquitin-protein ligase ZNR
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ZNRF3 antibody (orb1259198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using ZNRF3 antibody (orb1259198) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using ZNRF3 antibody (orb1259198) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver using ZNRF3 antibody (orb1259198) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using ZNRF3 antibody (orb1259198) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using ZNRF3 antibody (orb1259198) at dilution of 1:100 (40x lens).