ZNF318 Polyclonal Antibody

• Catalog Number: orb1421141
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ZNF318.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat
• Conjugation: Unconjugated
• UniProt ID: Q5VUA4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Zinc finger protein 318, Endocrine regulatory prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lymph node (Mouse) Lysate at 40 ug. Testis (Mouse) Lysate at 40 ug. A549 (Human) Cell Lysate at 30 ug. HL60 (Human) Cell Lysate at 30 ug. 293T (Human) Cell Lysate at 30 ug. Primary: Anti- ZNF318 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 125 kD. Observed band size: 125 kD.

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ZNF318) Polyclonal Antibody, Unconjugated at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.