ZNF225 rabbit pAb Antibody

SKU: orb766609

Description

Rabbit polyclonal antibody to ZNF225

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human ZNF225. AA range:641-690
• Molecular Weight: 82
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-ZNF225 antibody, anti-Zinc finger protein 225 antibody

Western Blot analysis of various cells using ZNF225 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunohistochemical analysis of paraffin-embedded Human thymus gland. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from HeLa cells, using ZNF225 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HT-29 cells using ZNF225 antibody.

Quick Database Links

UniProt

Q9UK10