ZNF225 rabbit pAb

• Catalog Number: orb766609
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ZNF225
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human ZNF225. AA range:641-690
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 82
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-ZNF225 antibody, anti-Zinc finger protein 225
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of various cells using ZNF225 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunohistochemical analysis of paraffin-embedded Human thymus gland. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from HeLa cells, using ZNF225 Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HT-29 cells using ZNF225 antibody.