ZLN024

SKU: orb1308912

Description

ZLN024 is an allosteric AMPK activator that stimulates active heterotrimers and inactive α1 subunit truncations (1-394 and 1-335), but not the 1-312 truncation. This small molecule is a valuable research tool for investigating AMPK signaling in metabolic and aging-related studies, applicable in both cellular and biochemical assays.

Research Area

Epigenetics & Chromatin, Signal Transduction

Key Properties

• CAS Number: 723249-01-2
• MW: 325.22
• Purity: 99.75% (May vary between batches)
• Formula: C₁₃H₁₃BrN₂OS
• SMILES: Cc1ccc(OCCSc2ncccn2)c(Br)c1
• Target: AMPK
• Solubility: DMSO:50 mg/mL (153.74 mM)

Bioactivity

• Target IC50:
  AMPK α2β2γ1:0.13 μM (EC50)|AMPK α1β1γ1:0.42 μM (EC50)|AMPK α1β2γ1:1.1 µM|AMPK α2β1γ1:0.95 μM (EC50)
• In Vivo:
  C57BKS db/db mice are administered a 15 mg/kg/day dose of ZLN024 by daily gavage for 5 weeks; 250 mg/kg/day Metformin (Met) is used as a positive control. After 4 weeks of treatment, ZLN024 improves glucose tolerance, reduces fasting blood glucose by 15%, and decreases liver tissue weight, triacylglycerol, and total cholesterol content.
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• In Vitro:
  ZLN024 allosterically stimulates active AMPK heterotrimers and the inactive α1 subunit truncations α1 (1-394) and α1 (1-335) but not α1 (1-312). It increases the activity of α1β1γ1 by 1.5-fold with an EC50 of 0.42 μM, α2β1γ1 by 1.7-fold with an EC50 of 0.95 μM, recombinant AMPK α1β2γ1 by 1.7-fold with an EC50 of 1.1 μM, and AMPK α2β2γ1 by 1.6-fold with an EC50 of 0.13 μM.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, AMP-activated protein kinase, AMPK α1β1γ1, AMPK α1β2γ1, AMPK α2β1γ1, AMPK α2β2γ1, AMPK, ZLN024, ZLN-024, ZLN 024

Compound Identifiers

PubChem CID

1825716