ZIP14 Antibody

• Catalog Number: orb1238541
• Category: Antibodies
• Description: ZIP14 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: ZIP14 antibody was raised against a 16 amino acid synthetic peptide near the center of human ZIP14.The immunogen is located within amino acids 230 - 280 of ZIP14.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 54 kDa Observed: 52 kDa
• Target: Slc39a14
• UniProt ID: Q15043
• NCBI: NP_001128625
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: ZIP14 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ZIP14 Antibody: NET34, ZIP14, cig19, LZT-Hs4, KIAA
• Note: For research use only
• Application notes: ZIP14 antibody can be used for detection of ZIP14 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of ZIP14 in Human Kidney. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ZIP14 antibody (orb1238541) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of ZIP14 in Mouse Colon. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ZIP14 antibody (orb1238541) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of ZIP14 in Rat Kideny. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ZIP14 antibody (orb1238541) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

WB Validation in Human, Mouse and Rat Tissues. Loading: 10 µg of lysate Antibodies: ZIP14, orb1238541, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.