Zinc Protoporphyrin

SKU: orb1218747

Description

Zinc Protoporphyrin is an orally active and competitive inhibitor of heme oxygenase-1 (HO-1) and markedly attenuates the protective effects of Phloroglucinol (PG) against H2O2, and has anti-cancer activity.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 15442-64-5
• MW: 626.02
• Purity: >98% (HPLC)
• Formula: C₃₄H₃₂N₄O₄Zn
• SMILES: OC(CCC1=C(C=C2[N]3=C4C(C)=C2CCC(O)=O)[N-]([Zn+2]35[N]6=C7C=C(C(C=C)=C8C)[N-]5C8=C4)C(C=C6C(C=C)=C7C)=C1C)=O
• Target: Endogenous Metabolite
• Solubility: DMSO:50 mg/mL (79.87 mM; Need ultrasonic);H2O:< 0.1 mg/mL (insoluble)

Bioactivity

• In Vivo:
  Animal model: BALB/c mice inoculated with C-26 cells. Dosage: 12.5, 25, 50 mg/kg for i.p.; 12.5, 50 mg/kg for p.o. Administration: IP or PO; from day 7 to 19. Result: Exerted dose-dependent antitumor effects manifested by the retardation of tumor growth.
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• In Vitro:
  Apoptosis Analysis Cell line: C-26 cells. Concentration: 5 μM. Incubation time: 72 hours. Result: The fraction of late apoptotic and necrotic cells increased from 10.9% in controls to 30.4% after 72 h. Cell Cytotoxicity Assay Cell line: C-26 and MDA-MB231 cells. Concentration: 1.25, 2.5, 5, 10, 20, 40 μM Incubation time: 48 or 72 hours. Result: Exerted cystostatic/cytotoxic effects against tumor cells. Cell Cycle Analysis Cell line: C-26 cells. Concentration: 2.5, 5 μM Incubation time: 48 or 72 hours. Result: Resulted in dose- and time-dependent reduction of cells in G1 phase of the cell cycle. Western blot analysis. Cell line: C-26 cells. Concentration: 1.25, 2.5, 5, 10, 20, 40 μM Incubation time: 48 hours. Result: Leaded to accumulation of cleaved (active) caspase-3.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Zn(II)-protoporphyrin IX | ZnPP | Zinc Protoporphyrin-9

Quick Database Links

Gene Symbol

Endogenous Metabolite