ZHX3 Antibody

SKU: orb2637268

Description

Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs). These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, FACS, IP, WB
• Dilution Range: ELISA (order BSA-free format for coating),Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
• Reactivity: Human
• Application Notes:
  Optimal dilution of the ZHX3 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: PCRP-ZHX3-1D11
• Immunogen: Recombinant full-length human ZHX3 protein was used as the immunogen for the ZHX3 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q9H4I2